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. 2022 May 2;8:240. doi: 10.1038/s41420-022-00890-0

Fig. 3. Knockdown of LINC00680 repressed the proliferation and ECM degradation.

Fig. 3

A LINC00680 knockdown (sh-LINC00680) was transfected into chondrocytes treated with IL-1β (10 ng/ml). RT-qPCR detected the level of LINC00680 upon sh-LINC00680 trasnfection. B CCK-8 proliferation assay detected the absorbance (450 nm) of chondrocytes treated with IL-1β and/or sh-LINC00680/sh-NC. C EdU (5′-ethynyl-2′-deoxyuridine) assay was performed for the proliferation of chondrocytes. EdU-positive cells and total Hoechst33258-positive cells were shown. D RT-PCR detected the mRNA levels of MMP-13 and Col2a1 in chondrocytes treated with IL-1β and/or sh-LINC00680/sh-NC. E Western blot analysis detected the MMP-13 protein and Col2a1 protein levels. Experiments’ data were repeated in triplicate. *Indicates the p < 0.05, and **Indicates the p < 0.01.