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. 2022 May 2;8:240. doi: 10.1038/s41420-022-00890-0

Fig. 5. LINC00680 positively regulated SIRT1 mRNA via IGF2BP2.

Fig. 5

A Subcellular fractionation location was performed to detect the RNA distribution of LINC00680. B Biotin-labeled RNA pull down was performed in LINC00680 overexpressing transfection. Probes targeting LINC00680 and its antisense RNA were synthesized. β-actin was used as a negative control. C Confocal microscopy for fluorescent in situ hybridization (FISH) showed the nuclear/cytoplasm fractionation of LINC00680 and IGF2BP2 using specific probes. Nuclei were stained blue (DAPI). D RIP-qPCR analysis of the enrichment of SIRT1 mRNA precipitated by IGF2BP2 relative to IgG in LINC00680 knockdown or control. E RNA stability analysis revealed the SIRT1 mRNA stability in chondrocytes when administrated with Act D (2 mg/mL), including IGF2BP2 overexpression and control. Experiments’ data were repeated in triplicate. *Indicates the p < 0.05. **Indicates the p < 0.01.