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. 2022 Mar 18;32(5):451–460. doi: 10.1038/s41422-022-00633-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Cryo-EM reconstruction of the IR of X. laevis NPC at 22 Å resolution. Two perpendicular views are shown. The inner diameter of the IR is ~45 nm. b The final EM reconstruction for the IR subunit displays an average resolution of 4.2 Å. Shown here is a curve of the Fourier Shell Correlation (FSC) over resolution. c Distribution of the local resolution for the EM reconstruction of the IR subunit. The color-coded resolution bar is shown below.