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. 2022 May 2;13:2367. doi: 10.1038/s41467-022-30089-x

Fig. 4. Role of NPTX2 in chronic itch-like behavior.

Fig. 4

a Scratching behavior for 24 hr in WT and NPTX2 KO mice before (Pre) and after DCP treatment (Post) (n = 11 mice/group; two-way repeated-measures ANOVA with post hoc Bonferroni test). b, c Dermatitis score (c) and transepidermal water loss (TEWL) (d) in DCP-treated WT and NPTX2 KO mice (n = 11 mice/group). d Scratching bouts induced by intradermal injection of chloroquine (200 μg) and compound 48/80 (50 μg) in WT and NPTX2 KO mice (chloroquine: WT, n = 7; KO, n = 5; compound 48/80: WT, n = 6; KO, n = 6). e Photograph of the cervical DRG (C3) removed after the microinjection with AAV-ESYN-GFP including blue dye. Scale bar, 1 mm. f GFP fluorescence in the cervical DRG (C3) of mice with AAV-ESYN-GFP. Scale bar, 100 μm. g Scratching behavior by the left hind limb for 24 hr in NPTX2 KO mice with AAV-ESYN-GFP (n = 6) or AAV-ESYN-NPTX2 (n = 7) before (Pre) and after DCP treatment to the left back (Post). Two-way repeated-measures ANOVA with post hoc Bonferroni test. h Scratching behavior by the left hind limb for 24 hr in WT mice with AAV-ESYN-GFP (n = 8) or AAV-ESYN-dnNPTX2 (n = 8) before (Pre) and after DCP treatment to the left back (Post). Two-way repeated-measures ANOVA with post hoc Bonferroni test. Values represent mean ± S.E.M. Source data are provided as a Source Data file.