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. 2022 May 2;5:407. doi: 10.1038/s42003-022-03358-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b A single A549 cell stained with SiR-actin after 14 h and 48 h of TGFβ1 addition respectively. c, d Extracted stress fiber image from (a, b), respectively. e, f Angular distribution and OOP values of the cells shown in (a, b), respectively. g Plot of OOP values of a live cell (shown in (a–f)) against time of TGFβ1 treatment (red). Multiple live-cell OOP trajectories (gray) against the time of TGFβ1 treatment. Scale bar: 16 μm.