REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
GFP-Trap® agarose beads	ChromTek	Cat# gta-20; RRID:AB_2631357
Rabbit polyclonal anti-giantin	Abcam	Cat# ab24586; RRID:AB_448163
Mouse monoclonal anti-vinculin	Sigma-Aldrich	Cat# V9131; RRID:AB_477629
Rabbit polyclonal anti-GFP	Abcam	Cat# ab6556; RRID:AB_305564
Mouse monoclonal anti-tubulin	Life Technologies	Cat# 13-8000; RRID:AB_2533035
Rabbit polyclonal anti-exoc6	Sigma-Aldrich	Cat# HPA036285; RRID:AB_10673617
Rabbit polyclonal anti-exoc6	Abcam	Abcam Cat# ab104200; RRID:AB_10716450
Mouse monoclonal anti-β1 integrin	Santa Cruz	Cat# sc-59829; RRID:AB_782090
Rabbit polyclonal anti-sortilin	Abcam	Cat# ab16640; RRID:AB_2192606
Monoclonal mouse anti-Rab33b	Abcam	Cat# ab57794; RRID:AB_945116
Monoclonal mouse anti-histidine	AbD serotec	Cat# MCA1396; RRID:AB_322084
Monoclonal mouse anti-GM130	BD Transduction Laboratories	Cat# 610822; RRID:AB_398141
Monoclonal mouse anti-Exo70	Santa Cruz	Cat# sc-365825; RRID:AB_10843358
Mouse monoclonal anti-Sec10	Santa Cruz	Cat# sc-514802; RRID:AB_2891091
Goat anti-Mouse IgG(H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488	Invitrogen	Cat# A-21121; RRID:AB_2535764
Goat anti-Rabbit IgG(H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488	Invitrogen	Cat# A27034; RRID:AB_2610664
Goat anti-Mouse IgG(H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594	Invitrogen	Cat# A-21155; RRID:AB_2535785
Goat anti-Rabbit IgG(H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594	Invitrogen	Cat# A-11012; RRID:AB_2534079
Goat anti-Mouse IgG(H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647	Invitrogen	Cat# A-21240; RRID:AB_2535809
Goat anti-Rabbit IgG(H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647	Invitrogen	Cat# A-21244; RRID:AB_2535812
Bacterial and virus strains
Escherichia coli BL21 (DE3)	Agilent Technologies	Cat# 200131
Chemicals, peptides, and recombinant proteins
DAPI ((2-(4-Amidinophenyl)-6-indolecarbamidine)	Sigma-Aldrich	Cat# D9542
Rhodamine-conjugated phalloidin	Invitrogen	Cat# R415
Lipofectamin 2000	Life Technologies	Cat# 10696153
Lipofectamin RNAiMax	Life Technologies	Cat# 13778150
His-dynabeads®	Thermo Fisher	Cat# 10103D
D-biotin	Sigma-Aldrich	Cat# B4501
Fibronectin	Sigma	Cat# F2006
Critical commercial assays
Yeast two-hybrid screen	Hybrigenics services	N/A
Experimental models: Cell lines
U-2 OS	ATCC	Cat# HTB-96; RRID:CVCL_0042
hTERT RPE-1	ATCC	Cat# CRL-4000; RRID:CVCL_4388
Oligonucleotides
siRNA targeting sequence: Rab33b (siRNA_1)	Dharmacon	Cat# J-008909-07
siRNA targeting sequence: Rab33b (siRNA_2)	Dharmacon	Cat# J-008909-08
siRNA targeting sequence: Rab33b (siRNA_3)	Dharmacon	Cat# J-008909-06
siRNA targeting sequence: Rab33b (siRNA_4)	Dharmacon	Cat# J-008909-09
Control siRNA: ACU UCG AGC GUG CAU GGC UTT	MWG-Biotech	N/A
siRNAs used in the RNAi primary screen, see Table S1	Dharmacon	N/A
Recombinant DNA
pEGFP-C1 vector	BD Bioscience Clontech	Cat# 6084-1
pEGFP-C1 Rab33b wt	GenScript	N/A
pEGFP-C1 Rab33b Q92L	GenScript	N/A
pEGFP-C1 Rab33b T47N	GenScript	N/A
pcDNA3.1-N-myc Rab33b wt	GenScript	N/A
pET-16b Rab33b wt	GenScript	N/A
pET-16b Rab33b Q92L	GenScript	N/A
pET-16b Rab33b T47N	GenScript	N/A
pET-16b Rab9 wt	Progida et al., 2012	N/A
pET-16b Rab9 Q66L	This paper	N/A
pCAG-mCherry-C-Sec15A	Kazuhisa Nakayama. Katoh et al., 2015	N/A
RFP-vinculin	Harald Stenmark. Lobert and Stenmark, 2012	N/A
Vinculin-GFP	Xian Hu	N/A
mApple-Integrin-β1	Addgene (from Michael Davidson)	Addgene plasmid # 54914; RRID:Addgene_54914
YFP-VSV-G	Susanne Pfeffer. Espinosa et al., 2009	N/A
BFP-Rab33b	This paper	N/A
Ii-STR-TNF-SBP-EGFP	Addgene (from Franck Perez). Boncompain et al., 2012	Addgene plasmid # 65280; RRID:Addgene_65280
Software and algorithms
ImageJ	https://imagej.nih.gov/ij/	N/A
GraphPad Prism 9	GraphPad Software Inc., https://www.graphpad.com	N/A
IncuCyte Zoom software analysis	Essen bioscience	N/A