Fig. 4. Glibenclamide decreased ISO-induced ROS generation in cardiomyocytes.

a Mouse cardiomyocytes were pretreated with glibenclamide (100 μmol/L) for 30 min and then treated with ISO for 30 or 120 min. The average ROS signal intensity was measured after DHE staining. b Mouse cardiomyocytes were treated with ISO for 30 min after 1–100 μmol/L glibenclamide pretreatment for 30 min. The average ROS signal intensity was measured after DHE staining. Gb, glibenclamide; ISO, isoprenaline. n = 6; scale bars: 100 μm; **P < 0.01; ***P < 0.001. One-way ANOVA with Tukey’s post hoc test