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. 2022 May 2;13:2386. doi: 10.1038/s41467-022-29840-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Bowman (rph3) compared to its near-isogenic line BW746 (Rph3). a Segments of infected leaves of cv. Bowman (top) and BW746 (bottom) at 2, 4, and 8 dpi. The scale bar is 0.5 cm. The photos were taken from the same leaves throughout the timepoints. The experiment was repeated five times with similar results. b Microscopic visualization of WGA-FITC-stained fungal colonization of mesophyll cells of cv. Bowman and BW746 leaves at 2, 4, and 8 dpi. The scale bar is 200 µm. c Quantification of P. hordei growth in cv. Bowman and BW746 leaves by the wheat germ agglutinin chitin assay. Fluorescence values for cv. Bowman are shown as blue dots; those from BW746 shown as orange dots. Values represent means ± SD (shown as the error bars) (n = 4).