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. 2022 May 2;13:2386. doi: 10.1038/s41467-022-29840-1

Fig. 3. Transcript levels of Rph3 detected by RT-qPCR during 2–12 dpi in response to virulent and avirulent pathotypes.

Fig. 3

The Rph3 gene was upregulated when the leaf was infected by Rph3-avirulent P. hordei pathotypes (green dot = 200 P−, green square = 5453 P+), whereas the transcript levels were unchanged when the leaf was infected by Rph3-virulent pathotypes (yellow cross = 5656 P+, yellow asterisk = 5457 P+) and P. triticina (blue diamond = 26-0, blue triangle = 104-1,2,3,(6),(7),11,13). The transcript levels of Rph3 in un-inoculated seedlings (mock inoculation) is shown in the black line. Values represent means ± SE (shown as the error bars) (n = 3). Samples inoculated with pathotype 5453 P+ at two dpi were used as calibrations to calculate the relative quantification (RQ) values using the delta-delta method with RQ = 2−∆∆Cq. The ADP-ribosylation factor gene was used as a normalizer.