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. 2022 May 2;13:2386. doi: 10.1038/s41467-022-29840-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Transient expression of executor resistance genes (Xa10, Xa27, Xa23, and Bs4C) and Rph3 (C-terminal HA-tagged) induce cell death in N. benthamiana under a mas promoter. b Non-synonymous Rph3 mutants M167 (L93F) and M181 (P126L) retained the ability to cause cell death in N. benthamiana, whereas the truncation mutant M198 (E72*) did not. Xa23 and Xa27 were used as controls for induction of cell death. The experiment was performed three times and included infiltration of two leaves of three to four plants with similar results.