Fig. 2.

Nsd2 deficiency results in reduced Tregs at the maternal–fetal interface and in fetal resorption. a Representative flow cytometric analysis of YFP⁺ Treg cells in the decidua of Nsd2^{Treg WT} and Nsd2^{Treg KO} mice previously mated with male BALB/c mice on Days E13.5–E14.5. b Percentage of CD44⁺CD62L^low and Helios⁺ Tregs determined by flow cytometry in the decidua of Nsd2^{Treg WT} and Nsd2^{Treg KO} mice previously mated with male BALB/c mice on Days E13.5–E14.5. c Flow cytometric analysis of IFNγ and IL-17A expression in decidual CD4⁺ T cells. n = 10. d Representative image of resorption of allogeneic embryos in the uterus of Nsd2^{Treg WT} and Nsd2^{Treg KO} mice on Day E14.5. Arrows indicate resorptions. e Percentage of resorbed embryos in all Nsd2^{Treg WT} and Nsd2^{Treg KO} pregnancies resulting from matings with male BALB/c mice. Two-sided Fisher’s exact test was used to assess the significance. f Percentage of resorption observed in individual mothers with the indicated genotype. g Incidence of pregnancies with at least one resorption. Two-sided Fisher’s exact test was used to assess the significance. h Histopathological evaluation of placentas from female Nsd2^{Treg WT} (left) and Nsd2^{Treg KO} (right, two examples) mice mated with male BALB/c mice; low-magnification survey of representative H&E-stained sections of placenta. D decidua, T trophoblast, L labyrinths. Scale bars, 1000 mm. i Representative Doppler image of the uterine artery (left, E14.5), which was used to determine the uterine artery resistance and pulsatility indices (right) of Nsd2^{Treg WT} and Nsd2^{Treg KO} female mice on Days E14.5 and E9.5. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001