Fig. 3.

Nsd2 deficiency did not affect the apoptosis or proliferation of decidual Tregs. a Cell-surface staining for Annexin V on decidual Tregs isolated from Nsd2^{Treg WT} and Nsd2^{Treg KO} mice. n = 5. b Flow cytometric analysis of Ki67 expression in decidual Tregs from Nsd2^{Treg WT} and Nsd2^{Treg KO} mice. n = 8. c Histogram overlay of CTLA-4, LAG-3, GITR, Nrp1, ICOS, OX40 and PD-1 expression and plots of the PD-1 (n = 3) mean fluorescence intensity (MFI) of decidual Treg cells from Nsd2^{Treg WT} and Nsd2^{Treg KO} mice. ***P ≤ 0.001; ns not significant