Fig. 6. Cytotoxic stimuli facilitates the release of degraded RNA m⁶A nucleoside to extracellular fluid and induces the misincorporated DNA 6 mA in neibourghing cells.

a, b The release of m⁶A (a) or rA (b) nucleoside from glioblastoma cells after H₂O₂ treatment. Glioblastoma cells were treated for 24 h with or without 1 mM H₂O₂, and then supernatants were collected and subjected to LC-MS/MS analysis. c Flow diagram of stable isotope labeling for tracing genomic incorporation. d Quantification of genomic DNA [D₃]-6mA in in glioma cell lines, GSCs, and glial cell line. The cells were treated with [D₃]-m⁶A at the concentration as indicated. (◊ indicated ND (not detected)).