Table 1.

Summary of fluorescent peptide-based probes for GzmB.

Compound	Amplification mechanism	Kcat (s−1)	KM (µM)	Kcat/KM (M−1 s−1)	LoD	Ref.
4-mer
Ac-IEPD-AMC	Fluorogenic dye	0.5	160	3.3 × 103	25 nM	Ref. 39
PEGylated IONPs	Nanoconstruct	~0.002	~0.2	1.1 × 104	n.d.	Ref. 25
CyGbPF	Fluorogenic dye	0.07	22.7	3.1 × 103	n.d.	Ref. 26
T1	FRET	2.3	30.8	5.9 × 104	17 nM	This work
6-mer
H5	FRET	117	9.6	1.2 × 107	6 pM	This work
9-mer
qTJ71	FRET	0.65	9.9	6.6 × 105	n.d.	Ref. 27