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. 2022 Apr 13;25(5):104249. doi: 10.1016/j.isci.2022.104249

Figure 3.

Figure 3

L3MBTL2 represses CGA by mediating histone modifications

(A) Overexpression of L3MBTL2 correlates with increased H2AK119ub1, HDAC1, and H3K27me3 at the CGA promoter but does not affect H3K9me3 and H3K36me3. ChIP of IgG control antiserum, HDAC1, H3Ac, H3K27me3, H3K9me3, and H3K36me3 (left to right) followed by qPCR analysis of CGA in PANC-1 cells.

(B) Schematic of dCas9 fusion constructs, dCas9-USP16, dCas9-EP300, dCas9-HDAC3, dCas9-KDM6B, and different gRNA constructs.

(C, D, and F), Relative mRNA levels of CGA in L3MBTL2-overexpressing PANC-1 cells co-transfected with either dCas9 fusion proteins (dCas9-USP16 (C), dCas9-EP300 (D), and dCas9-KDM6B (F)) or dCas9 empty vector and the indicated gRNAs.

(E) Relative mRNA levels of CGA in L3MBTL2 knockdown PANC-1 cells co-transfected with either dCas9-HDAC3 or dCas9 empty vector and the indicated gRNAs. Data are presented as means ± SD (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, t-tests.