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. 2022 Apr 13;25(5):104249. doi: 10.1016/j.isci.2022.104249

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© 2022 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

L3MBTL2, MAX, and RING1B synergistically inhibit CGA expression

(A) The proximal CGA promoter contains three E-box elements. The plasmid containing the wild-type sequence of the CGA promoter (WT) or mutations in different E-boxes (MUT) was co-transfected with either the pcDNA3.1-L3MBTL2 plasmid or the pcDNA3.1 empty vector. Data are presented as the relative Luc activity of CGA promoters in the presence of exogenous L3MBTL2 as a percentage of the activity of the same reporter in transfections with the control vector. Data are presented as means ± SD (n = 3).

(B) In vitro Pup(E) modification assay of L3MBTL2 overexpressed cells co-transfected with dxCas3.7-PafA and gRNAs. Western blots showed Pup(E) modification of L3MBTL2, MAX, and RING1B.