Figure 4: Anopheles stephensi hairy, odd-skipped and runt are not expressed progressively.

Whole-mount in situ hybridization of hairy (h) (a-g), odd-skipped (odd) (h-n), and runt (run) (o-s) from early cellular blastoderm to the end of germband extension. During early blastoderm, h stripes begin as faint stripes (a) and quickly resolve into six pair-rule stripes (b) that are six-cells wide (c). (c) represents an inset of the region indicated in (b). Seven h stripes are complete at the end of the blastoderm stage (d). An eight stripe is added as germband elongation begins (e). Partway through germband elongation, the anterior stripes fade but stripes 6, 7, and 8 remain (f). At the end of germband elongation, h is expressed in the head and at the posterior end of the germband (g). odd expression begins in a broad central domain (h) but five pair-rule stripes rapidly appear (i). These stripes are three-cells wide at their emergence (j). (j) represents an inset of the region indicated in (i). odd stripes 6 and 7 are detectable by the end of blastoderm stage, as well as a small domain at the posterior tip (k and arrowhead). At gastrulation, secondary stripes (asterisks) begin to intercalate between the pair-rule stripes (l). Both segmental and posterior tip odd expression patterns continue through germband elongation (m-n). run is initially expressed as a broad domain that spans the trunk of the blastoderm (o). This pattern continues through blastoderm (p-q) and gastrulation (r). By the end of germband elongation run transitions to a messy segmental pattern (s). Images are shown in order of increasing time. All embryos are depicted from a lateral view with the anterior on the left side.