TABLE 1.
Top ten phosphorylation sites predicted to be most differentially phosphorylated by CK2WT and CK2K198R in axonally localized human ion channels.
| Protein name | Position | Sequence | CK2WT probability (%) | CK2K198R probability (%) | Probability difference | Known phosphorylation site |
|---|---|---|---|---|---|---|
| SCN1A | 510 | KRKQKEQS*GGEEKDE | 34 | 100 | 66% | Paralog to known SCN2A S514 site |
| SCN2A | 514 | KKKQKEQS*GEEEKND | 35 | 100 | 65% | b Yes, Human |
| KCNA1 | 438 | VSSPNLAS*DSDLSRR | 64 | 23 | −40% | b Yes, Human |
| SCN2A | 1,126 | TEEFSSES*DMEESKE | 83 | 44 | −39% | a Yes, Human |
| CACNA1H | 1,027 | AEGDANRS*DTDEDKT | 64 | 25 | −39% | No |
| CACNA1D | 1,266 | FKPKGYFS*DAWNTFD | 61 | 23 | −39% | No |
| SCN1A | 1,136 | TEDFSSES*DLEESKE | 83 | 44 | −38% | Paralog to known SCN2A S1126 site |
| CACNA1H | 2,094 | GGEEAEAS*DPADEEV | 61 | 24 | −37% | No |
| CACNA1D | 2,138 | QDFGPGYS*DEEPDPG | 81 | 44 | −37% | No |
| KCNQ2 | 839 | PYIAEGES*DTDSDLC | 58 | 22 | −36% | b Yes, Mouse |
a
Sites were considered to be known if they appeared on PhosphoSitePlus.
b
Sites were identified in fewer than three high throughput experiments.