Fig. 34. (A) Schematic illustration of the rGO-aptamer-RCA based sensing probe. (B) Analysis of RCA products using 0.6% agarose gel electrophoresis. Each reaction was conducted at 30 °C for 1 h in 60 μL of target binding buffer (4,5,6,7-tetrabromobenzotriazole (TBB), 20 mM PBS, 150 mM NaCl, 20 mM KCl, and 5 mM MgCl2 at pH 7.5) having components of rGO-adsorbed functional thrombin probe TP1 (250 nM), circular DNA template (CDT1) (8 nM), and thrombin (Thr; 200 nM). (C) Time-dependent fluorescence response of rGO-adsorbed FAM-labeled TP1 (250 nM) in the presence of Thr (200 nM), CDT1 (8 nM), or both. Excitation wavelength (λex)/emission wavelength (λem) = 494 nm/518 nm. [Reprinted with permission from ref. 716, M. Liu, J. Song, S. Shuang, C. Dong, J. D. Brennan and Y. A. Li, Graphene-Based Biosensing Platform Based on the Release of DNA Probes and Rolling Circle Amplification, ACS Nano, 2014, 8, 5564–5573. Copyright© American Chemical Society.] (D) Illustration of the GO-based platform coupled with hybridization chain reactions (HCR) for biothiol analysis. [Reprinted with permission from ref. 724, J. Ge, Z.-M. Huang, Q. Xi, R.-Q. Yu, J.-H. Jiang and X. Chu, A Novel Graphene Oxide Based Fluorescent Nanosensing Strategy with Hybridization Chain Reaction Signal Amplification for Highly Sensitive Biothiol Detection, Chem. Commun., 2014, 50, 11879–11882. Copyright© Royal Society of Chemistry.].
