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. 2022 Apr 19;10:845118. doi: 10.3389/fcell.2022.845118

FIGURE 1.

FIGURE 1

Workflow of MOSAIC and overview of compartmental states. (A) MOSAIC workflow starts with an individual intra-chromatin O/E matrix. After two rounds of SVD, potential centromere effect is removed from the matrix, and the two eigenvectors that can best reflect the structural features are selected for K-means clustering. Finally, a modified Louvain algorithm is applied to refine the results of K-means clustering. (B) The average compartmentalization strength of all chromosomes versus the average modularity of all chromosomes for the top-ten EVs in terms of modularity for each chromosome. (C,D) Pearson correlation coefficients between the top-ten EVs sorted by modularity and H3K27ac (C) and H3K27me3 (D), respectively. (E,F) Enrichment of H3K27ac (E) and H3K27me3 (F) for all 100 kb bins of chromosome 1 in relation to EV1 and EV2, respectively. The enrichment values are ZSCOR-normalized. (G) Compartmental states of all 100 kb bins of chromosome 1 in relation to EV1 and EV2. (H) Gene expression for the A2 and B2 regions identified by the MOSAIC and A/B compartment schemes (p value calculated by one-tailed Mann–Whitney rank test). (I) Hi-C correlation heatmap of Chr1: 0–100 Mb along with EV1, EV2, H3K27me3, H3K27ac, and compartmental states. (J) Hi-C correlation heatmap of Chr1: 27–37, 60–70 Mb and the distal region Chr1: 190–208 Mb.