TABLE 1.

Oligonucleotide primer sequences for the amplification of the entire ndh gene^a

Primer	Description	Sequence	Annealing temp (°C)	PCR product size (bp)
ndh1S	First fragment, sense	5′GCT AAC TGA ACT CGC TCA TC	55	356
ndh1AS	First fragment, antisense	5′AAT TCC GAG ACG ACG CAC TG
ndh2S	Second fragment, sense	5′GCA ATG TCC AGG TAC TGT TG	57	387
ndh2AS	Second fragment, antisense	5′CCT TGG TCG AGT CGA TGT G
ndh3S	Third fragment, sense	5′GAC AGA TCG CCG AGC TGG C	60	372
ndh3AS	Third fragment, antisense	5′TGG ACA GGT CGG GCA GCA C
ndh4S	Fourth fragment, sense	5′GGA CCT TGC CGA GCA ATC AC	60	351
ndh4AS	Fourth fragment, antisense	5′CAG GTA CGC CAG GTG CAG CA
ndh5S	Fifth fragment, sense	5′CCA AGA TCG GTC CCG TTG AG	59	292
ndh5AS	Fifth fragment, antisense	5′ACT GAG TAC CTG GCA GGC TG

^aThe M. tuberculosis sequence used to design the primers was obtained from GenBank, accession number Z83859.