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. 2022 Apr 19;10:875155. doi: 10.3389/fcell.2022.875155

TABLE 1.

List of primers used in qPCR.

Gene GenBank ID Forward primer Reverse primer
ASCL1 NM_204412.1 5ʹ-CCC​GGC​AGG​ACG​CTC-3ʹ 5ʹ-GGG​GAG​AGG​AAA​ACG​CAA​CA-3ʹ
E2F1 NM_205219.1 5ʹ-GGA​CGA​TCT​CAT​CCA​GAC​GTG-3ʹ 5ʹ-ACG​TAG​GCT​GCG​TGC​T-3ʹ
MITF NM_205029.1 5ʹ-CCC​AAA​TCA​AAC​GAC​CCG​GAT​A-3ʹ 5ʹ-GTG​CGT​TGC​TGC​TCT​CTT​TG-3ʹ
OTX2 NM_204520.2 5ʹ-GTC​GGT​TAT​CCC​GCC​ACC-3ʹ 5ʹ-TTT​TCA​AGG​CCA​CCT​CCT​CC-3ʹ
PAX6 NM_205066.1 5ʹ-GGC​AGA​AGA​TCG​TGG​AAC​TC-3ʹ 5ʹ-TTC​GTA​ATA​CCT​GCC​CAA​AA-3ʹ
RPE65 NM_204884.1 5ʹ-CCT​ACC​ACC​GGA​GGT​TTG​TT-3ʹ 5ʹ-GGT​CTG​GGT​AGG​CGT​AGG​TA-3ʹ
RPLP0 NM_204987.2 5ʹ-GGA​GCT​CAC​AGC​TCG​TCT​TT-3ʹ 5ʹ-TAG​TTG​GAC​TTC​CAC​GTC​GC-3ʹ
SIX6 NM_001389365.1 5ʹ-AGG​TGG​GCA​ACT​GGT​TCA​AA-3ʹ 5ʹ-CTG​CTG​CTG​TAG​CCT​GTT​CT-3ʹ
SOX2 NM_205188.2 5ʹ-TGA​ACG​GAT​CGC​CTA​CCT​AC-3ʹ 5ʹ-CTG​GAT​TCC​GTC​TTG​ACC​AC-3ʹ
TYR NM_204160.1 5ʹ-TTT​GCT​GAT​CCA​CAC​ACT​GC-3ʹ 5ʹ-GAT​CAT​TCG​CAG​AGC​CTT​GT-3ʹ
VSX2 NM_204768.1 5ʹ-CAG​ACG​GCC​AGC​TCA​GAT​TC-3ʹ 5ʹ-AGG​CCT​TTT​CCA​GCT​CTT​CC-3ʹ