Table 1.
UPLC-MS/MS parameters used for the quantitation and the confirmation of 20 pharmacologically active dyes in water.
| Analyte | Retention time (min) | Precursor ion (m/z) | Product ion (m/z) | DP (V) | CE (V) | CXP (V) | Dwell time (ms) |
|---|---|---|---|---|---|---|---|
| malachite green (MG) | 2.14 | 329.3 | 313.2 208.4 |
50 50 |
51 46 |
20 12 |
20 |
| crystal violet (CV) | 2.34 | 372.2 | 356.2 340.5 |
50 50 |
54 71 |
21 19 |
20 |
| brilliant green (BG) | 2.68 | 385.5 | 341.3 297.1 |
50 50 |
52 72 |
21 17 |
15 |
| ethyl violet (EV) | 3.33 | 456.8 | 412.3 368.3 |
46 46 |
62 81 |
20 20 |
15 |
| methyl violet 2B (MV) | 2.09 | 358.5 | 342.3 326.4 |
44 44 |
52 71 |
22 20 |
20 |
| pararosaniline (PR) | 1.55 | 288.2 | 194.8 168.1 |
57 57 |
44 54 |
18 9 |
20 |
| victoria blue B (VBB) | 2.33 | 470.3 | 454.1 349.2 |
100 100 |
58 52 |
12 22 |
5 |
| victoria blue R (VBR) | 2.32 | 422.3 | 406.2 393.2 |
110 110 |
51 54 |
10 23 |
20 |
| victoria pure blue BO (VPBBO) | 2.85 | 478.2 | 434.4 390.3 |
115 115 |
59 76 |
10 9 |
20 |
| methylene blue (MB) | 1.67 | 284.2 | 268.2 252.2 |
35 35 |
48 71 |
15 15 |
20 |
| azure A (AZA) | 1.55 | 256.2 | 213.8 199.1 |
38 38 |
46 58 |
12 11 |
20 |
| azure B (AZB) | 1.60 | 270.0 | 254.1 212.1 |
72 72 |
47 67 |
15 14 |
20 |
| azure C (AZC) | 1.50 | 242.1 | 199.9 227.0 |
34 34 |
47 38 |
12 12 |
15 |
| thionine (TH) | 1.45 | 228.2 | 195.9 210.8 |
33 33 |
41 42 |
10 12 |
20 |
| new methylene blue (NMB) | 1.87 | 312.3 | 283.2 254.0 |
82 82 |
41 51 |
13 15 |
20 |
| nile blue A (NBA) | 1.89 | 318.3 | 274.0 260.0 |
74 74 |
47 71 |
16 13 |
15 |
| acriflavine (ACR) | 1.50 | 224.2 | 208.9 181.9 |
35 35 |
38 49 |
12 17 |
20 |
| proflavine (PRO) | 1.48 | 210.1 | 192.9 165.8 |
25 25 |
39 46 |
11 9 |
20 |
| rhodamine B (RB) | 1.88 | 443.2 | 399.1 355.1 |
90 90 |
58 80 |
12 12 |
5 |
| rhodamine 6G (R6G) | 2.19 | 442.2 | 415.3 341.1 |
105 105 |
47 65 |
12 12 |
20 |
| malachite green- d5 (MG-d5) | 2.13 | 334.4 | 318.2 | 50 | 51 | 19 | 20 |