Table 2.

Validation results for the UPLC-MS/MS method for the determination of 20 pharmacologically active dyes in environmental water.

Analyte	LOD (μg/l)	LOQ (μg/l)	Concentration level (μg/l)	Recovery (%)	Precision (RSD, %)	Intermediate precision (RSD, %)
MG	0.003	0.01	0.01	102.1	5.4	9.8
			0.05	100.6	4.8	8.6
			0.1	101.6	4.4	8.2
CV	0.003	0.01	0.01	99.3	3.9	6.9
			0.05	100.0	4.8	7.6
			0.1	103.1	6.0	8.5
BG	0.003	0.01	0.01	98.7	7.8	9.2
			0.05	99.2	7.5	9.8
			0.1	98.4	8.4	10.3
EV	0.03	0.1	0.1	98.7	12.8	13.2
			0.5	99.8	11.5	12.7
			1.0	103.4	10.3	11.6
MV	0.003	0.01	0.01	99.8	6.5	7.3
			0.05	101.4	6.8	7.9
			0.1	102.3	5.2	7.4
PR	0.003	0.01	0.01	73.5	6.5	10.5
			0.05	71.2	5.4	9.8
			0.1	75.4	5.0	9.6
VVB	0.03	0.1	0.1	102.1	7.4	9.5
			0.5	104.9	8.1	10.3
			1.0	103.7	7.0	9.9
VBR	0.03	0.1	0.1	98.3	4.7	6.8
			0.5	96.3	5.6	7.0
			1.0	95.5	6.0	7.5
VPBBO	0.03	0.1	0.1	96.0	7.8	10.5
			0.5	102.7	7.0	9.1
			1.0	101.7	6.9	9.5
MB	0.03	0.1	0.1	97.5	6.7	8.9
			0.5	95.5	8.5	9.7
			1.0	102.8	9.0	10.5
AZA	0.03	0.1	0.1	101.1	10.0	11.5
			0.5	100.0	8.8	10.8
			1.0	99.3	7.9	10.2
AZB	0.03	0.1	0.1	97.2	8.8	11.0
			0.5	100.2	9.5	11.7
			1.0	98.6	8.0	10.7
AZC	0.03	0.1	0.1	97.3	6.7	8.9
			0.5	102.2	7.6	9.7
			1.0	95.3	8.9	10.2
NMB	0.003	0.01	0.01	99.8	10.0	11.4
			0.05	89.9	8.9	10.7
			0.1	90.9	9.7	11.0
TH	0.03	0.1	0.1	102.3	7.9	10.1
			0.5	100.1	8.5	11.5
			1.0	99.1	9.9	12.2
NBA	0.03	0.1	0.1	100.3	7.6	8.9
			0.5	94.8	7.1	8.1
			1.0	97.8	8.6	10.1
ACR	0.03	0.1	0.1	100.2	7.9	9.2
			0.5	98.9	6.5	8.3
			1.0	100.1	6.9	9.9
PRO	0.03	0.1	0.1	99.9	6.5	8.1
			0.5	96.7	5.4	7.6
			1.0	103.2	5.1	7.4
RB	0.003	0.01	0.01	102.3	7.8	9.9
			0.05	100.4	8.9	10.2
			0.1	97.6	9.1	10.7
R6G	0.003	0.01	0.01	95.4	7.4	8.8
			0.05	98.9	7.0	8.3
			0.1	97.2	6.8	7.8