Fig. 4. The interaction between HOTAIRM1 and miR-148b in AML cells. (A) The WT or MUT fragments of HOTAIRM1 carrying the predicted miR-148b binding sites. (B) HL60 cells were cotransfected with WT-HOTAIRM1 or MUT-HOTAIRM1 and miR-148b or miR-con for 48 h, and luciferase activity was then measured by luciferase reporter assay. (C) qRT-PCR analysis was performed to detect miR-148b expression in HL60 and THP-1 cells transfected with si-HOTAIRM1 or si-con for 48 h. *P < 0.05.