Testosterone levels and cytotoxicity of compounds 15–21 in TM3 cells^a.

Compd	R2	R3	Testosterone Levelsb (μM/L)	IC50c (μM)
7		—	271.7 ± 10.0	>100
15		—	282.2 ± 5.5	>100
16		—	188.5 ± 5.5	>100
17		—	303.5 ± 5.5	>100
18		—	237.0 ± 4.0	>100
19		—	222.8 ± 7.0	>100
20		—	227.1 ± 3.0	>100
21		O	259.5 ± 6.5	>100

^aSee Experimental section.

^bTestosterone levels in the culture medium are measured by ELISA kit; data are expressed as mean ± SD of three independent assays.

^cActivities of compounds against the growth of TM3 cells are detected with the SRB assay and their IC₅₀ data are an average of at least three independent experiments.