Fig. 7.

Fluorescence staining of cells cultured on the cell culture control polystyrene (PS) and on ceramic scaffolds (TCP and TMP-D/-P). Cells, which grew on the well plate bottom (W) and on the scaffold (S) were analyzed separately. F-actin is stained in red, cell nuclei are stained in blue. Cells showing actin ring formation, a minimum of 3 nuclei, and a cell size >50 μm were classified as active osteoclasts. These mature osteoclasts could be verified from day 10 onwards for PS, for TCP at day 16 and 22, and for TMP-D and –P for day 22. Mononuclear osteoclasts were detected on TMP-D after 16 days and on TMP-P after 22 days (labelled with an arrow). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)