Table 1.
List of RT-qPCR primers to detect specific crypt cell type
| Gene | Cell marker | Forward | Reverse | Amplicon size |
|---|---|---|---|---|
| Lgr5 | Stem cells | GACAATGCTCTCACAGAC | GGAGTGGATTCTATTATTATGG | 162 bp |
| Olfm4 | Stem cells | GCCACTTTCCAATTTCAC | GAGCCTCTTCTCATACAC | 199 bp |
| Axin2 | Stem cells | GGACTGGGGAGCCTAAAGGT | AAGGAGGGACTCCATCTACGC | 334 bp |
| Lrig | Stem cells | TTCCTTACCGGTGAGACTGG | CCATCACTGTGCCAACACTT | 190 bp |
| Lyz1 | Paneth cells | GGAATGGATGGCTACCGTGG | CATGCCACCCATGCTCGAAT | 290 bp |
| Defa5 | Paneth cells | TATCTCCTTTGGAGGCCAAG | TTTCTGCAGGTCCCAAAAAC | 120 bp |
| Ki67 | Transit amplifying cells | ATCATTGACCGCTCCTTTAGGT | GCTCGCCTTGATGGTTCCT | 104 bp |
| Tbp | Normalization gene | ATCCCAAGCGATTTGCTG | CCTGTGCACACCATTTTTCC | 91 bp |
| Rpl13 | Normalization gene | GTGGTCCCTGCTGCTCTCAAG | CGATAGTGCATCTTGGCCTTTT | 152 bp |