REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD24-Pacific Blue (1/500)	BioLegend	Cat # 01829
Epcam-APC (1/500)	Abcam	Cat # ab95641
Rabbit anti-GFAP (1/200)	DAKO	Cat # Z0334
Alexa fluor 488 goat anti-rabbit (1/500)	Thermo Fisher Scientific	Cat # A-11078
Chemicals, peptides, and recombinant proteins
Propidium iodide (1/1000)	Invitrogen	Cat # P1304MP
Matrigel	Corning	Cat # CACB356231
EGF	Sigma-Aldrich	Cat # E9644
Noggin	Cedarlane labs	Cat # 6057NG
R-spondin-1	RnD	Cat # 3474RS
GDNF	PeproTech	Cat # 450-44
Cell Recovery Solution	Corning	Cat # C354253
TrypLE express	Thermo Fisher Scientific	Cat # 12604013
DMEM-F12	Thermo Fisher Scientific	Cat # 21331020
N-2 Supplement	Thermo Fisher Scientific	Cat # 17502-048
B-27 Supplement, serum free	Thermo Fisher Scientific	Cat # 17504-044
GlutaMAX	Thermo Fisher Scientific	Cat # 35050-038
HEPES (1 M)	Thermo Fisher Scientific	Cat # 15630-056
N-Acetyl-L-cysteine	Thermo Fisher Scientific	Cat # A9165
Penicillin-Streptomycin	Thermo Fisher Scientific	Cat # 15140130
Poly-D-Lysine	Sigma-Aldrich	Cat # P6407-5MG
Laminin	Invitrogen	Cat # 23017015
EDTA	Invitrogen	Cat # 15575020
Gentamicin	Thermo Fisher Scientific	Cat # 15750060
Hanks’ Balanced Salt Solution (HBSS)	Thermo Fisher Scientific	Cat # 14175095
Sucrose	Sigma-Aldrich	Cat # S8501
Sorbitol	Sigma-Aldrich	Cat # S1876
PBS 1× (no calcium, no magnesium)	Thermo Fisher Scientific	Cat # 10010023
Fetal Bovine Serum (FBS)	Thermo Fisher Scientific	Cat # 26140087
Hoechst 33342 (1/5000)	Thermo Fisher Scientific	Cat # 1799639
Critical commercial assays
SuperScript® III enzyme	Invitrogen	Cat # 18080093
RNeasy® Plus Mini Kit	QIAGEN	Cat # 741344
RNeasy® Plus Micro Kit	QIAGEN	Cat # 74034
Power SYBR green master mix	Applied Biosystems	Cat # 4368706
Experimental models: Organisms/strains
Lgr5-eGFP mouse line: adult (6–10 weeks), male or female	The corresponding lab	Barker et al. (2007)
Oligonucleotides
RT-qPCR primers	Baghdadi et al. (2021)	Table 1
Software and algorithms
FlowJo	BD Biosciences	https://www.flowjo.com
Prism	Prism	http://www.graphpad.com
ImageJ	Schneider et al. (2012)	https://imagej.nih.gov/ij/download.html
Others
Clear polystyrene sterile certified non-pyrogenic 12-well plate	VWR	Cat # 10062-894
Clear polystyrene sterile certified non-pyrogenic 24-well plate	VWR	Cat # 10062-896
0.22 μm pore size syringe filter	TPP	Cat # 99722
1 mL syringe Plastipak	BD	Cat # 305959
15 mL conical tube Falcon	BD	Cat # 352097
50 mL conical tube Falcon	BD	Cat # 352098
RNase Away	Thermo Fisher Scientific	Cat # 7002
Flow cytometer	No recommended vendor	N/A
Nanodrop photospectrometer	Thermo Fisher Scientific	Cat # ND-2000