Table 3.
Associations of binary markers with recurrence, ER, PR, her2 status and binary ki67 (above median 12 or below median 12), results from bivariate association analysis by the Fisher's exact test and logistic regression with adjustments for the effects of age and TILs density (>45% or≤45%).
| Recurrence |
|||||
|---|---|---|---|---|---|
| No | Yes | P [1] | Odds ratio [95% C.I.] | p.adj [2] | |
| N | 54 | 13 | |||
| CD8 > 70 (%) | 17 (31.5) | 9 (69.2) | 0.024 | 1.54 [0.26, 8.83] | 0.622 |
| CD4 > 90 (%) | 21 (38.9) | 10 (76.9) | 0.027 | 1.93 [0.35, 11.72] | 0.449 |
| CD68 > 50 (%) | 13 (24.5) | 10 (76.9) | 0.001 | 5.79 [1.32, 31.56] | 0.026 |
| CD163 > 70 (%) | 23 (42.6) | 9 (69.2) | 0.123 | 1.58 [0.37, 7.14] | 0.532 |
| FOXP3 > 6 (%) | 24 (45.3) | 11 (84.6) | 0.014 | 2.84 [0.42, 23.94] | 0.287 |
| CD8/FOXP3 ratio >5 (%) | 25 (47.2) | 2 (15.4) | 0.057 | 0.56 [0.06, 5.15] | 0.587 |
| CD68/CD163 ratio >0.46 (%) |
22 (41.5) |
12 (92.3) |
0.001 |
16.73 [2.63, 338.79] |
0.013 |
| ER | |||||
| negative |
positive |
P [1] |
Odds ratio [95% C.I.] |
p.adj [2] |
|
| N | 13 | 54 | |||
| CD8 > 70 (%) | 9 (69.2) | 17 (31.5) | 0.024 | 0.14 [0.02, 0.80] | 0.033 |
| CD4 > 90 (%) | 11 (84.6) | 20 (37.0) | 0.004 | 0.06 [0.01, 0.37] | 0.006 |
| CD68 > 50 (%) | 4 (30.8) | 19 (35.8) | 1.00 | 2.32 [0.50, 12.76] | 0.301 |
| CD163 > 70 (%) | 7 (53.8) | 25 (46.3) | 0.76 | 1.03 [0.25, 4.30] | 0.969 |
| FOXP3 > 6 (%) | 11 (84.6) | 24 (45.3) | 0.014 | 0.15 [0.02, 0.86] | 0.044 |
| CD8/FOXP3 ratio >5 (%) | 2 (15.4) | 25 (47.2) | 0.057 | 3.73 [0.63, 29.92] | 0.161 |
| CD68/CD163 ratio >0.46 (%) |
9 (69.2) |
25 (47.2) |
0.218 |
0.38 [0.09, 1.46] |
0.175 |
| PR | |||||
| negative |
positive |
P [1] |
Odds ratio [95% C.I.] |
p.adj [2] |
|
| N | 16 | 51 | |||
| CD8 > 70 (%) | 10 (62.5) | 16 (31.4) | 0.039 | 0.23 [0.04, 1.08] | 0.068 |
| CD4 > 90 (%) | 13 (81.2) | 18 (35.3) | 0.002 | 0.07 [0.01, 0.37] | 0.003 |
| CD68 > 50 (%) | 5 (31.2) | 18 (36.0) | 1.00 | 2.12 [0.52, 10.09] | 0.316 |
| CD163 > 70 (%) | 8 (50.0) | 24 (47.1) | 1.00 | 1.22 [0.34, 4.60] | 0.758 |
| FOXP3 > 6 (%) | 13 (81.2) | 22 (44.0) | 0.011 | 0.17 [0.03, 0.81] | 0.032 |
| CD8/FOXP3 ratio >5 (%) | 3 (18.8) | 24 (48.0) | 0.045 | 3.25 [0.68, 18.26] | 0.148 |
| CD68/CD163 ratio >0.46 (%) |
11 (68.8) |
23 (46.0) |
0.154 |
0.38 [0.10, 1.29] |
0.132 |
| her2 | |||||
| negative |
positive |
P [1] |
Odds ratio [95% C.I.] |
p.adj [2] |
|
| N | 48 | 12 | |||
| CD8 > 70 (%) | 18 (37.5) | 8 (66.7) | 0.104 | 0.93 [0.14, 5.65] | 0.942 |
| CD4 > 90 (%) | 22 (45.8) | 8 (66.7) | 0.333 | 0.53 [0.06, 3.34] | 0.511 |
| CD68 > 50 (%) | 16 (34.0) | 6 (50.0) | 0.334 | 0.77 [0.15, 3.48] | 0.734 |
| CD163 > 70 (%) | 22 (45.8) | 8 (66.7) | 0.333 | 1.21 [0.26, 5.72] | 0.803 |
| FOXP3 > 6 (%) | 24 (50.0) | 10 (83.3) | 0.052 | 1.54 [0.17, 14.26] | 0.684 |
| CD8/FOXP3 ratio >5 (%) | 21 (43.8) | 2 (16.7) | 0.107 | 1.30 [0.11, 29.67] | 0.837 |
| CD68/CD163 ratio >0.46 (%) |
23 (48.9) |
7 (58.3) |
0.748 |
0.98 [0.23, 4.15] |
0.973 |
| ki67 [3] | |||||
| ≤12 |
>12 |
P [1] |
Odds ratio [95% C.I.] |
p.adj [2] |
|
| N | 33 | 29 | |||
| CD8 > 70 (%) | 6 (18.2) | 20 (69.0) | <0.001 | 6.05 [1.48, 27.67] | 0.014 |
| CD4 > 90 (%) | 10 (30.3) | 20 (69.0) | 0.005 | 2.34 [0.61, 8.85] | 0.206 |
| CD68 > 50 (%) | 7 (21.9) | 16 (55.2) | 0.009 | 2.58 [0.71, 9.67] | 0.148 |
| CD163 > 70 (%) | 12 (36.4) | 19 (65.5) | 0.041 | 2.06 [0.62, 6.79] | 0.23 |
| FOXP3 > 6 (%) | 10 (30.3) | 24 (82.8) | <0.001 | 6.28 [1.60, 27.61] | 0.01 |
| CD8/FOXP3 ratio >5 (%) | 18 (54.5) | 6 (20.7) | 0.009 | 0.54 [0.13, 2.16] | 0.381 |
| CD68/CD163 ratio >0.46 (%) | 13 (40.6) | 19 (65.5) | 0.073 | 2.37 [0.74, 7.94] | 0.148 |