FIGURE 2.

PMS reduced the percentage of GRK6‐positive neurons in ACC. (A) Immunofluorescence study showed the co‐localization of GRK6 with NeuN, GFAP, or Iba1 in ACC of CON and PMS rats (Bar = 50 μm). The enlarged pictures in the right panel were to show the extent of co‐localization of GRK6 (green) with NeuN (red), GFAP (red), or Iba1 (red) (Bar = 5 μm) and the yellow staining indicated the co‐localization. (B) Data analysis showed that GRK6 was primarily present in NeuN positive neurons with a small amount in GFAP‐positive astrocytes and Iba1‐positive microglial cells in ACC. Statistical analysis indicated that the ratio of GRK6‐positive cells in NeuN positive cells in the ACC region of PMS rats were significantly decreased when compared with CON rats (n = 3 for each group, *p < 0.05, two‐tailed two‐sample t‐test)