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. 2022 Mar 29;28(6):851–861. doi: 10.1111/cns.13827

FIGURE 4.

FIGURE 4

Upregulation of P2Y6 expression in ACC contributed to visceral hypersensitivity of PMS rats. (A) The mRNA level of P2Y6 was significantly increased in ACC of PMS rats compared with CON rats (n = 4 for each group, *p < 0.05, two‐tailed two‐sample t‐test). (B) Compared with CON rats, P2Y6 total protein expression was significantly increased in ACC of PMS rats (n = 8 for PMS rats, n = 9 for CON rats, *p < 0.05, two‐tailed two‐sample t‐test). (C) Compared with CON rats, P2Y6 membrane protein expression was significantly increased protein in ACC of PMS rats (n = 10 for PMS rats, n = 8 for CON rats, ***p < 0.001, two‐tailed two‐sample t‐test). (D) The ratio of P2Y6 membrane protein expression over P2Y6 total protein expression was significantly increased (Total protein level: n = 8 for PMS rats, n = 9 for CON rats; Membrane protein level: n = 10 for PMS rats, n = 8 for CON rats, compared with CON rats, **p < 0.01, two‐tailed two‐sample t‐test)