FIGURE 3.

(A) Schematic drawing of inhibition of the GABA_B receptor‐mediated signaling pathway in the shrew PONV model. In shrews showing emetic behaviors, expression of the ADCY1 gene, which encodes adenyl cyclase (orange), was increased, whereas expression of the GABBR1 and CACNB1 genes, which encode GABAB receptor R1 subunit (green) and voltage‐gated calcium channel (blue), was decreased. Administration of pharmacological baclofen (a GABA_B receptor agonist) eliminated emetic behaviors in the shrew PONV model. (B) Expression levels of the GABBR1, ADCY1, and CACNB1 genes in whole‐transcriptome sequencing. Bar graphs and error bars indicate means and standard errors of the means. The gene expression of GABBR1 and CACNB1 was not observed in the Surgery group. FPKM value: fragments per kilobase of exon per million mapped fragments for quantifying the assembled transcript expression