Fig. 4.

H₂S stimulates DosS and DosR phosphorylation. (A) Representative autoradiogram of PAGE-resolved recombinant DosS following autophosphorylation in the presence of γ-³²P-ATP alone (left and center lanes) or with γ-³²P-ATP in the presence of 100 μM Na₂S (right lanes). (B) Densitometric quantitation of DosS bands (n = 3) at 60 min. (C) Representative autoradiogram of PAGE-resolved recombinant DosS and DosR following phosphorylation of DosR by γ-³²P-labeled DosS alone (left and center lanes) or with γ-³²P-labeled DosS in the presence of 100 μM Na₂S (right lanes). These reactions were performed by adding γ-³²P-labeled ATP to a reaction containing both DosS and DosR and analyzed at different time points. (D) Densitometric quantitation of DosR bands (n = 3) at 60 min. Data in (B) and (D) are shown as the mean $\pm$ SEM and were analyzed using one-way ANOVA with Tukey's multiple comparisons test performed using GraphPad Prism version 9. **p < 0.01. Autoradiograms are representative of at least 3 independent assays.