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. Author manuscript; available in PMC: 2022 May 3.
Published in final edited form as: ACS Infect Dis. 2020 Sep 14;6(10):2719–2731. doi: 10.1021/acsinfecdis.0c00341

Figure 6. Role of cardiolipin in NDM-1 binding to the outer bacterial membrane.

Figure 6.

(a) Distance evolution of NDM-1 vs. outer membrane distance during CG MD simulations, with and without CDLs. One representative replica for each membrane model is reported. (b) Lipid contacts between the enzymes and the different lipid types that compose the bilayer, normalized according to lipid composition of the membrane (left); lipid distribution in proximity of NDM-1 and N-VIM, averaged over all the MD trajectories (right); (c) SDS-PAGE analysis of sucrose gradient fractions from liposome flotation assays of NDM-1 C26A, using liposomes with an outer membrane composition (+CDL) or liposomes with an equivalent composition but lacking cardiolipin (−CDL).