Figure 11.

Binding of imipenem (solid lines) or cefotaxime (dashed lines) to apo-BcII (gray lines) and to Zn(II)-BcII (black lines) followed by Trp fluorescence. The absence of changes in the fluorescence of apo-BcII upon substrate addition reveals the lack of binding to this variant. Reproduced with permission from reference²³⁹ Rasia et al. Copyright 2004 American Society for Biochemistry and Molecular Biology.