Figure 35.

Photodiode array stopped-flow spectra of di-Co(II) BcII showing changes in the ligand-to-metal charge transfer (LMCT, 343 nm) and ligand field bands (d-d, 500–650 nm) during the hydrolysis of benzylpenicillin. The inset shows an amplification of the ligand field bands (in the 450–700 nm region of the spectra). The arrows indicate whether there is an increase or a decrease in the intensity of the corresponding absorption bands in the transition from an ES complex to the resting state enzyme. Adapted with permission from reference²⁸⁷ Llarrull et al. Copyright 2008 American Chemical Society.