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Spectral changes during hydrolysis of imipenem by bi-Co(II) BcII, monitored by stopped-flow absorbance meassurements. The absorption at 408 nm is due to the anionic intermediate, obscuring the Cys-Co(II) LMCT band. The inset shows changes in the ligand field band during turnover, that reveal modifications in geometry of the Co(II) center. Adapted with permission from reference240 Tioni, Llarrull, Vila and coworkers. Copyright 2008 American Chemical Society.
