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Fig. 4. (a) Confocal microscopy images of actin and fascin-1 in control BMDCs and LPS-treated BMDCs. Nuclei, actin, and fascin-1 in DCs were stained with DAPI (blue), phalloidin (green), and mouse anti-fascin-1 antibody(red), respectively. (b) Migratory path of cells without dendrites (blue, purple) and cells with dendrites (red, orange). (c) Migration rate of DCs were calculated using the MATLAB CellTracker plugin. Data are expressed as mean values ± SD. ***P < 0.001.