FIG. 4.

Inclusion morphology. (A and B) C. suis strain S45 formed typical inclusions when grown in the absence of TET. (C and D) C. suis strain R19 predominately formed typical inclusions when grown in the absence of TET. These inclusions are heavily laden with developmental forms. When grown in 2 μg of TET/ml, however, the majority of the inclusions contained only a few large aberrant RBs (E and F). Panels A, C, and E represent DIC micrographs; panels B, D, and F are fluorescent images labeled with antibodies against HSP60. Bar, 5 μm.