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. 2001 Aug;45(8):2198–2203. doi: 10.1128/AAC.45.8.2198-2203.2001

FIG. 7.

FIG. 7

C. trachomatis L2 and C. suis R19 growth within a single inclusion. HeLa cells sequentially infected with R19 (time, 0 h) and L2 (time, 16 h) were fixed with methanol and labeled with antibodies directed against L2 and R19. (A) DIC micrograph of an infected cell containing a single inclusion 50 h after infection with R19. (B) Fluorescent image of the cells shown in panel A labeled with antisera against L2 MOMP (red) and R19 MOMP (green). (C) DIC micrograph of infected cells fixed 32 h after infection with R19. (D) Fluorescent image of the cells shown in panel C labeled with antisera against L2 IncA (red) and R19 MOMP (green). The nuclei of cells shown in panel D are colored blue with 4′,6-diamino-2-phenylindole (Sigma). Bar, 5 μm.