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. Author manuscript; available in PMC: 2022 May 3.
Published in final edited form as: Biochim Biophys Acta. 2012 Jan 12;1817(5):792–801. doi: 10.1016/j.bbabio.2012.01.004

Figure 1. The absence of PSI in the PSI deleted ΔAB mutant strain of Synechocystis 6803 is confirmed by the lack of P700 oxidation-induced absorbance change at 703 nm in the ΔAB membrane preparation.

Figure 1

(A) Absorption transient at 703 nm reflecting the P700 oxidation and re-reduction kinetics of the WT Synechocystis 6803 membranes and lack of it in the ΔAB membranes. (B) Absorption transient at 703 nm (on an expanded scale) demonstrating the lack of the P700 response in the ΔAB membranes as compared to that of the ΔAB membranes with 1%, 2% and 5% WT admixtures. In both (A) and (B), the arrows indicate the times when the actinic flashes were fired. The reaction buffer contained 50 mM Tris-HCl (pH 8.3), 33 µM DCPIP, 1.7 mM sodium ascorbate and 50 nM PSII. The experiments were carried out at 24±1°C.