Fig. 3.

Characterization of IPO43 cell line in 2D model. The cell line presents cobblestone morphology or a pavement-like arrangement characteristic of epithelial cells in a confluent monolayer (cells with polygonal shape). A Representative morphological appearance of IPO43 cell line at passage 40, phase contrast microscopy, 100x; B Representative morphology of IPO43 cells at passage 80, phase contrast microscopy, 100x and 200x; C/D: Proliferation curve of IPO43 cell line, C Cell counting was determined by microscopy using the trypan blue exclusion method, and in D nuclei counting was determined by crystal violet staining, at regular time intervals (1 to 9 days) and E Wound healing assay to determine the migration rate of IPO43. Representative images of wound-healing assays performed in IPO43 cell. Cells were plated onto a 12 well dish and treated with mitomycin-C. Wound was generated and cells migrate filling the wound after 72 h. Pictures were taken at 0 h, 24 h, 48 h and 72 h after the scratch was performed (bright field microscopy), 100x