Fig. 8.

3D culture progression with measurement of aggregates area, growth curve and aggregation profile of IPO43 cells in the presence of ROCK Inhibitor in stirred-tank culture systems. A Aggregation of IPO43 with and without ROCK Inhibitor. Cells were cultured in a stirred-tank culture system placed on a magnetic stirrer (set at 40 rpm) and supplemented with and without ROCK Inhibitor, for 72 days. Supplementation with ROCK Inhibitor improved aggregation and after 72 h most aggregated cells are still viable, scale bar 100 µm. B IPO43 aggregates area and C Growth curve, determined by PicoGreen analysis. Values are presented as mean ± SD. Area increased with culture time, but growth tends to be lower