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. 2022 Apr 30;22:175. doi: 10.1186/s12935-022-02600-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 9 — Characterization and expression of biological markers relevant for the establishment of 3D cell culturing models in IPO43 cell line. A Cryosections of 3D aggregates stained with hematoxylin and eosin (HE) and by immunohistochemistry. Cells show positive expression for cytokeratin (CK) type II (clone CAM 5.2), vimentin and p53. Cells were negative for WT1 and had positive expression for CA 125. B Cryosections of 3D aggregates show positive expression for CK18, collagen IV and β-catenin. Only few cells show positive expression for vimentin, E-cadherin and Ki-67, 400x