FIG. 4.

In vitro culture strategies recapitulating osteocytic morphologies. (A) IDG-SW3 cells cultured in a 2D single layer, modified with permission from Yang et al.¹²⁵ (B) OmGFP66 cell line and the LCN, modified with permission from Wang et al.¹²⁴ (C) IDG-SW3 cells cultured within a 3D gelatin hydrogel, modified with permission from Yang et al.¹²⁵ (D) MLO-A5 cells cultured with biphasic calcium phosphate microparticles, modified with permission from Gu et al.¹³⁹ (E) Single BMSC cultured on a 3D tubular microisland, modified with permission from Ma et al.¹⁴¹ (F) Primary osteoblasts cultured within a 3D collagen fibrous scaffold, modified with permission from Matsugaki et al.¹⁴⁴ LCN, lacunocanalicular network.