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. Author manuscript; available in PMC: 2022 May 3.
Published in final edited form as: Cytometry A. 2020 Aug 21;99(4):372–381. doi: 10.1002/cyto.a.24193

Figure 2. Gating strategy for exosomes on beads and controls.

Figure 2.

In A, using of a forward scatter pulse analysis permits for selection of labeled single non-aggregated exosome-bead complexes and their discrimination from larger aggregates. In B, detection of OX40L on CD63+ exosomes isolated from plasma of a patient with HNC. capture used Biotinylated anti-CD63 Ab was used for capture. In C, controls necessary for on-bead flow cytometry measurements of OX40L on exosomes. Detection is with APC-labelled anti-Ox40L Ab.