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. Author manuscript; available in PMC: 2022 May 3.
Published in final edited form as: Cytometry A. 2020 Aug 21;99(4):372–381. doi: 10.1002/cyto.a.24193

Figure 3. Establishment of the separation index (SI).

Figure 3.

An example of flow cytometric stain of exosomes on beads gated on the main bead population visible on the forward/side scatter. Note, the low MFI of the isotype control compared to the MFI of the APC-labeled detection Ab (anti-OX40L Ab). Capture was with biotinylated anti-CD63 Ab. The SI calculated for this sample (1.8) is based on the difference in MFI between isotype control and Ab-stained exosomes on beads and the average spread of their distributions. The formula for calculating the SI is shown.