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. 2022 Mar 15;12(5):1075–1086. doi: 10.1002/2211-5463.13384

Fig. 1.

Fig. 1

The proposed N‐terminal MAP kinase (NtMAP) site is unique to NOS3. Alignment of the proposed NtMAP kinase binding site in human NOS3, to human NOS1 and NOS2 using T‐Coffee [27, 28]. The alignment of this subsection of NOS3 from 96‐115 to NOS1 and 2 is identical when aligned with all three full sequences or just when the subsequence is used against the full sequences of NOS1 and NOS2. The postalignment analysis of the sequences above was ‘good’ for all residues with the shown conservation [29]. The underlined segment is the interaction site proposed previously for JNK [10]. The MAP kinase interacting motif LVF (italics above)—ΦXΦ motif is minimally conserved in NOS1 and NOS2 providing evidence that it might also be a unique NOS3 interaction site. The unique NOS3 SP site is highlighted in gray. * ‐ exact conservation of amino acids, : ‐ conservation of amino acids with highly similar properties,. ‐ conservation of amino acids with weakly similar properties.