Fig. 4.
MAP kinases bind unphosphorylated but not phosphorylated NtMAP and AI peptides. BLI sensorgrams of analyte MAP kinases binding to ligand peptides. Ligand–analyte pairs are (A) NtMAP‐ERK2, (B) AI‐ERK2, (C) NtMAP‐p38α, (D) AI‐p38α, (E) NtMAP‐JNK1α1, (F) AI‐JNK1α1. Analyte concentrations in A‐F were 1000 nm (red), 500 nm (yellow), 250 nm (green), 125 nm (blue), 63 nm (orange), and 31 nm (purple). Raw data are rendered as points with fits to a global one‐state association‐then‐dissociation model shown in black. (G), Ligand NtMAP with phosphorylated S114 and H), Ligand AI with phosphorylated S633 evinced negligible binding to kinase analytes ERK2 (red), p38α (yellow), and JNK1α1 (green), indicating very low or no affinity.